| Autor: |
Yu Fan, Fen Liu, Li-yuan Fan, Cheng-yuan Mao, Han Liu, Chan Zhang, Yan-peng Yuan, Jing Yang, Yan-lin Wang, Yu-tao Liu, Chang-he Shi, Yu-ming Xu |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Stem Cell Research, Vol 52, Iss , Pp 102257- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2021.102257 |
| Popis: |
Here, we describe the generation of an induced pluripotent stem cell (iPSC) line, from a female patient diagnosed with Parkinson’s disease (PD). The patient carries a heterozygous intermediate-length GGC repeat expansions mutation in the NOTCH2NLC gene. Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver Klf4, OCT3/4, SOX2 and c-MYC factors. The generated iPSC line (ZZUi020-A) presented with expression of common pluripotency markers, showed potential of differentiating into derivatives of the three germ layers, and displayed a normal karyotype. The clone ZZUi020-A is presented thereafter, it can be used to study the mechanisms underlying NOTCH2NLC-PD pathogenesis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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