A customizable digital holographic microscope

Autor: Claudia Ravasio, Luca Teruzzi, Mirko Siano, Llorenç Cremonesi, Bruno Paroli, Marco A.C. Potenza
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: HardwareX, Vol 19, Iss , Pp e00569- (2024)
Druh dokumentu: article
ISSN: 2468-0672
DOI: 10.1016/j.ohx.2024.e00569
Popis: We propose a compact, portable, and low-cost holographic microscope designed for the characterization of micrometric particles suspended in a liquid. This system is built around a commercial optical microscope by substituting its illumination source (a light-emitting diode) with a collimated laser beam. Similarly, a quartz flow cell replaces the microscope glass slide using a 3D-printed custom mount. With the hardware presented in this paper, the holographic imaging of the electromagnetic fields emitted by the particles that intercept the laser beam achieves a resolution close to that of optical microscopes but with a greater depth of field. Several morphological and optical features can be extracted from the holograms, including particle projected section, aspect ratio, and extinction cross-section. Additionally, we introduce a remote system control that enables users to process the acquired holograms on a remote computational device. This work provides a comprehensive description of the methodology of image processing in holographic microscopy and a series of validation measurements conducted using calibrated particles. This technique is suitable for the characterization of airborne particles found in snow, firn, and ice; here we report experimental results obtained from Alpine ice cores.
Databáze: Directory of Open Access Journals