Development of an Antigen Capture Lateral Flow Immunoassay for the Detection of Burkholderia pseudomallei

Autor: Teerapat Nualnoi, Paweena Wongwitwichot, Siriluk Kaewmanee, Pornchanan Chanchay, Nattapong Wongpanti, Tossapol Ueangsuwan, Rattikarn Siangsanor, Wannittaya Chotirouangnapa, Tanatchaporn Saechin, Suwanna Thungtin, Jidapa Szekely, Chaiyawan Wattanachant, Vannarat Saechan
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Diagnostics, Vol 14, Iss 10, p 1033 (2024)
Druh dokumentu: article
ISSN: 14101033
2075-4418
DOI: 10.3390/diagnostics14101033
Popis: Early diagnosis is essential for the successful management of Burkholderia pseudomallei infection, but it cannot be achieved by the current gold standard culture technique. Therefore, this study aimed to develop a lateral flow immunoassay (LFIA) targeting B. pseudomallei capsular polysaccharide. The development was performed by varying nitrocellulose membrane reaction pads and chase buffers. The prototype LFIA is composed of Unisart CN95 and chase buffer containing tris-base, casein, and Surfactant 10G. The assay showed no cross-reactivity with E. coli, S. aureus, P. aeruginosa, and P. acne. The limit of detections (LODs) of the prototype LFIA was 107 and 106 CFU/mL B. pseudomallei in hemoculture medium and artificial urine, respectively. These LODs suggest that this prototype can detect melioidosis from positive hemoculture bottles but not straight from urine. Additionally, these LODs are still inferior compared to Active Melioidosis Detect (AMDTM). Overall, this prototype holds the potential to be used clinically with hemoculture bottles. However, further improvements should be considered, especially for use with urine samples.
Databáze: Directory of Open Access Journals
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