The Impact of Warm Ischemia Time on Kidney Function in Experiment

Autor: Ulugbek Abduganiev, Muhammadaziz Aliev, Pulat Sultanov
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: International Journal of Biomedicine, Vol 14, Iss 2, Pp 295-299 (2024)
Druh dokumentu: article
ISSN: 2158-0510
2158-0529
DOI: 10.21103/Article14(2)_OA10
Popis: Background: The main difficulty with donation after circulatory death is the inevitable period of warm ischemia, which may adversely affect tissue viability and graft function after transplantation. The aim of this study was to evaluate dynamic changes in the functional parameters of the kidneys because of renal warm ischemia (RWI) in the experiment. Methods and Results: The experiments were carried out on 78 white male rats weighing 214.5±31.8g. To achieve the study's aim, we applied a method of modeling the intraoperative RWI by clamping renal arteries from both sides through a median laparotomy under ether anesthesia. Vascular clamping lasted 12, 24, 36, or 48 minutes in four experimental groups of rats, each containing 18 rats. The intact group of rats became the control group (n=6). In each of the 4 experimental groups, rats were euthanized by decapitation on Days 3, 7, and 14 of the experiment. Before euthanasia, a 24-hour urine collection was performed in metabolic chambers. Laboratory tests included the determination of blood urea nitrogen (BUN), serum creatinine (sCR), serum potassium (SP), and urinary creatinine (uCr); glomerular filtration rate (GFR) was calculated using the Rehberg-Tareev method. Warm ischemia time (WIT) for no more than 12 minutes did not lead to significant negative changes in most of the studied parameters of renal function at all time stages of the experiment, with the exception of a significant decrease in GFR, as well as an increase in SP levels on Days 3 and 7 of the experiment. The WIT up to 24 minutes led to a more pronounced drop in GFR at all time stages of the experiment (P
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