| Autor: |
Lívia Maria Mendonça Augusto, Diego Pinheiro Aguiar, Danielle Cabral Bonfim, Amanda dos Santos Cavalcanti, Priscila Ladeira Casado, Maria Eugênia Leite Duarte |
| Jazyk: |
English<br />Portuguese |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Revista Brasileira de Ortopedia, Vol 51, Iss 1, Pp 70-74 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1982-4378 |
| DOI: |
10.1016/j.rboe.2015.12.013 |
| Popis: |
ABSTRACT OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
