Single nucleotide polymorphism (SNP) markers for genetic diversity and population structure study in Ethiopian barley (Hordeum vulgare L.) germplasm

Autor: Mihret Yirgu, Mulugeta Kebede, Tileye Feyissa, Berhane Lakew, Aemiro Bezabih Woldeyohannes, Mulusew Fikere
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: BMC Genomic Data, Vol 24, Iss 1, Pp 1-13 (2023)
Druh dokumentu: article
ISSN: 2730-6844
DOI: 10.1186/s12863-023-01109-6
Popis: Abstract Background High-density single nucleotide polymorphisms (SNPs) are the most abundant and robust form of genetic variants and hence make highly favorable markers to determine the genetic diversity and relationship, enhancing the selection of breeding materials and the discovery of novel genes associated with economically important traits. In this study, a total of 105 barley genotypes were sampled from various agro-ecologies of Ethiopia and genotyped using 10 K single nucleotide polymorphism (SNP) markers. The refined dataset was used to assess genetic diversity and population structure. Results The average gene diversity was 0.253, polymorphism information content (PIC) of 0.216, and minor allelic frequency (MAF) of 0.118 this revealed a high genetic variation in barley genotypes. The genetic differentiation also showed the existence of variations, ranging from 0.019 to 0.117, indicating moderate genetic differentiation between barley populations. Analysis of molecular variance (AMOVA) revealed that 46.43% and 52.85% of the total genetic variation occurred within the accessions and populations, respectively. The heat map, principal components and population structure analysis further confirm the presence of four distinct clusters. Conclusions This study confirmed that there is substantial genetic variation among the different barley genotypes. This information is useful in genomics, genetics and barley breeding.
Databáze: Directory of Open Access Journals
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