Autor: |
Jun WANG, Lin LIU, Yong GUO, Yong-hui WANG, Le ZHANG, Long-guo JIN, Rong-xia GUAN, Zhang-xiong LIU, Lin-lin WANG, Ru-zhen CHANG, Li-juan QIU |
Jazyk: |
angličtina |
Rok vydání: |
2014 |
Předmět: |
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Zdroj: |
Journal of Integrative Agriculture, Vol 13, Iss 9, Pp 1854-1864 (2014) |
Druh dokumentu: |
article |
ISSN: |
2095-3119 |
DOI: |
10.1016/S2095-3119(13)60579-1 |
Popis: |
Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines (RHL) derived from the cross between Yangyandou (low level of β subunit) and Zhonghuang 13 (normal level of β subunit) were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1 (β subunit content), which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome (Glyma 1.0, Williams 82), including the two copies of β subunit coding gene CG4. An InDel marker developed from a thymine (TT) insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection (MAS). |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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