| Autor: |
Shakti Kumar, Naina Kumari, Daizee Talukdar, Akansha Kothidar, Mousumi Sarkar, Ojasvi Mehta, Pallavi Kshetrapal, Nitya Wadhwa, Ramachandran Thiruvengadam, Bapu Koundinya Desiraju, G. Balakrish Nair, Shinjini Bhatnagar, Souvik Mukherjee, Bhabatosh Das, GARBH-Ini Study Group, Vineeta Bal, Sumit Misra, Uma Chandra Mouli Natchu, Satyajit Rath, Kanika Sachdeva, Dharmendra Sharma, Amanpreet Singh, Shailaja Sopory, Arindam Maitra, Partha P. Majumder, Tushar K. Maiti, Monika Bahl, Shubra Bansal, Umesh Mehta, Sunita Sharma, Brahmdeep Sindhu, Sugandha Arya, Rekha Bharti, Harish Chellani, Pratima Mittal, Anju Garg, Siddharth Ramji, Ashok Khurana, Reva Tripathi, Yashdeep Gupta, Smriti Hari, Nikhil Tandon, Rakesh Gupta, Dinakar M. Salunke, G Balakrish Nair |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2235-2988 |
| DOI: |
10.3389/fcimb.2021.622474 |
| Popis: |
BackgroundThe incidence of preterm birth (PTB) in India is around 13%. Specific bacterial communities or individual taxon living in the vaginal milieu of pregnant women is a potential risk factor for PTB and may play an important role in its pathophysiology. Besides, bacterial taxa associated with PTB vary across populations.ObjectiveConduct a comparative analysis of vaginal microbiome composition and microbial genomic repertoires of women who enrolled in the Interdisciplinary Group for Advanced Research on Birth Outcomes – A DBT India Initiative (GARBH-Ini) pregnancy cohort to identify bacterial taxa associated with term birth (TB) and PTB in Indian women.MethodsVaginal swabs were collected during all three trimesters from 38 pregnant Indian women who delivered spontaneous term (n=20) and preterm (n=18) neonates. Paired-end sequencing of V3-V4 region of 16S rRNA gene was performed using the metagenomic DNA isolated from vaginal swabs (n=115). Whole genome sequencing of bacterial species associated with birth outcomes was carried out by shotgun method. Lactobacillus species were grown anaerobically in the De Man, Rogosa and Sharpe (MRS) agar culture medium for isolation of genomic DNA and whole genome sequencing.ResultsVaginal microbiome of both term and preterm samples reveals similar alpha diversity indices. However, significantly higher abundance of Lactobacillus iners (p-value All_Trimesters |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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