DESIGNING OF MULTI-EPITOPE VACCINE CONSTRUCT EMPLOYING IMMUNOINFORMATICS APPROACH TO COMBAT MULTI-TICK SPECIES INFESTATIONS
| Autor: | A. Nandi, Manisha, V. Solanki, V. Tiwari, B. Sajjanar, M. Sankar, M. Saini, S. Shrivastava, S. K. Bhure, S. Ghosh |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Exploratory Animal and Medical Research, Vol 12, Iss 2, Pp 149-159 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2277-470X 2319-247X |
| DOI: | 10.52635/eamr/12.2.149-159 |
| Popis: | Ticks have an exceptional ability to parasitize diverse animal and human lives and cause direct losses by transmitting a wide variety of pathogens to their host. For the management of the tick a vaccine using mid-gut antigen, Bm86 was developed which provided a variable degree of efficacy against different strains of the cattle tick, Rhipicephalus microplus. Contrary to a single antigen-based vaccine, it is expedient to use multi-epitopes vaccine targeting multiple functions of ticks. To develop a universal tick vaccine against multi-species, multiepitopic construct using immunodominant conserved tick proteins with B and T-cell epitopes were designed employing various immuno-informatics techniques. The 3D structure of the final construct was predicted, refined, and validated by different computational techniques. Molecular docking of the construct was carried out with TLR-2 receptor to predict efficient cell mediate the immune response. The findings manifest that the multi-epitopic construct might be active against multi-tick species infestations, a problem faced by many tropical and subtropical countries. |
| Databáze: | Directory of Open Access Journals |
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