Detection of mutations in NOD2/CARD15 gene in Arab patients with Crohn's disease

Autor: Iqbal Siddique, Abu S Mustafa, Islam Khan, Ali H Ziyab, Munira Altarrah, Riyas Sulaiman, Numeer Kadungothayil, Faraz Shaheed
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: The Saudi Journal of Gastroenterology, Vol 27, Iss 4, Pp 240-248 (2021)
Druh dokumentu: article
ISSN: 1319-3767
1998-4049
DOI: 10.4103/sjg.sjg_582_20
Popis: Background: Mutations in NOD2/CARD15 gene have been linked to an increased risk of Crohn's disease (CD). The objective of this study is to determine NOD2/CARD15 gene mutations, and their association with the risk of CD in Arabs in Kuwait. Methods: Four NOD2 gene mutations, including Pro268Ser (SNP5), Arg702Trp (SNP8), Gly908Arg (SNP12), and Leu1007FsinsC (SNP13) were examined in Arab CD patients (n = 103) and control subjects (n = 100). The genomic DNA was isolated and used in polymerase chain reaction (PCR) with four sets of specific primers. The PCR-amplified DNA fragments were sequenced and analyzed for the NOD2 mutations. Logistic regression was used to estimate the adjusted odds ratios (aOR) and 95% confidence intervals (CI). Results: Of the four genotyped variants, the Arg702Trp (SNP8) and Leu1007FsinsC (SNP13) variants were not informative in our study sample due to minor allele frequency of
Databáze: Directory of Open Access Journals
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