| Autor: |
V. Ballandras, L. McNamara, J.C. Carolan, S. Byrne |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Irish Journal of Agricultural and Food Research, Vol 63, Iss 1, Pp 54-65 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2009-9029 |
| DOI: |
10.15212/ijafr-2023-0115 |
| Popis: |
Barley yellow dwarf viruses (BYDVs) belong to a complex of several species, all vectored by aphids. Due to the abundance of Sitobion avenae and Rhopalosiphum padi, BYDV-MAV and BYDV-PAS are among the prevalent species in Irish crops. Several BYDV detection methods, such as immunosorbent assays and PCR-based diagnostic tests, are available and routinely used. However, there are opportunities to develop improved assays to capture viral load information from different sample matrices. Here, we successfully developed a droplet digital PCR assay to detect and quantify BYDV-MAV and BYDV-PAS in both aphid and barley samples. The high specificity shown by this assay allows us to differentiate the two species from each other within a wide dynamic range. This assay will provide a better overview of the process underlying BYDV infection and transmission from the early stage of infection to the appearance of the symptoms. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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