Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells

Autor: Nazar Oleksiievets, Yelena Sargsyan, Jan Christoph Thiele, Nikolaos Mougios, Shama Sograte-Idrissi, Oleksii Nevskyi, Ingo Gregor, Felipe Opazo, Sven Thoms, Jörg Enderlein, Roman Tsukanov
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Communications Biology, Vol 5, Iss 1, Pp 1-8 (2022)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-021-02976-4
Popis: Oleksiievets et al. combine single-molecule localization super-resolution microscopy technique DNA-PAINT with fluorescence lifetime imaging to allow fast multi-target super-resolution imaging in one spectral region by separating two or three different targets by fluorescence lifetime of the associated dyes. The method is demonstrated on two complementary experimental techniques, a standard confocal laser scanning setup, and a widefield microscope employing a novel TCSPC camera as the detector.
Databáze: Directory of Open Access Journals
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