Silencing of LncRNA HULC enhances chemotherapy induced apoptosis in human gastric cancer
| Autor: | Zhang Yifei, Song Xiaojing, Hu Jinchen, Jiang Lixin |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Journal of Medical Biochemistry, Vol 35, Iss 2, Pp 137-143 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1452-8258 1452-8266 |
| Popis: | Background: Gastric cancer (GC) is one of the most common cancers in the world; however, chemoresistance greatly decreases the efficacy of therapy in gastric cancer. Long noncoding RNAs (IncRNAs) participate in a variety of biological processes, and we hypothesize that lncRNA H ULC regulates the multidrug resistance in GC treatment. Methods: We obtained GC tissue samples from 42 GC patients and detected the expression level of HULC in the plasma and tissues via qRT-PCR. The relationship between HULC expression and survival rate was confirmed by Kaplan-Meier survival analysis. We verified the expression of H U LC in GC cell lines via qRT-PCR, and the function of HULC was detected via flow cytometry assay and CCK-8 assay. Results: HULC was highly expressed in the plasma and tissues of the GC patients compared with controls, with HULC high expression indicating lower survival rate. HULC knockdown enhanced cisplatin-induced apoptosis in GC cells. Conclusions: Our results suggest that silencing lncRNA HULC could enhance chemotherapy induced apoptosis in GC cells, which could provide a novel approach for therapeutic strategies. |
| Databáze: | Directory of Open Access Journals |
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