Mitigation of Ochratoxin A-Induced Renal Toxicity by Kaempferol in Albino Mice
| Autor: | Norah Fahad Saad Bosaad, Peramaiyan Rajendran, Rebai Ben Ammar, Gamal Mahmoud Bekhet, Mohammed Salem Moqbel, Saeed Yaseen Al-Ramadan, Hamad Abu Zahra |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Research Journal of Pharmacognosy, Vol 11, Iss 3, Pp 53-65 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2345-4458 2345-5977 |
| DOI: | 10.22127/rjp.2024.441154.2348 |
| Popis: | Background and objective: This study investigated the protective effects of kaempferol, a natural flavonoid known for its antioxidant properties, against ochratoxin A-induced renal damage. Ochratoxin A, a nephrotoxic mycotoxin found in contaminated food and feed, primarily induces renal damage through oxidative stress. The objective was to assess kaempferol’s potential in mitigating ochratoxin A -induced renal toxicity and its underlying molecular mechanisms. Methods: Male albino mice were divided into four groups: normal control, kaempferol control, ochratoxin A -induced renal toxicity, and ochratoxin A -induced renal toxicity treated with kaempferol. Ochratoxin A was administered twice a week for 8 weeks at 5 mg/kg body weight to induce renal toxicity. Kaempferol (50 mg/kg) was administered twice a week for 8 weeks after a 4-hour interval following ochratoxin A administration. Results: Results showed that kaempferol normalized levels of blood urea nitrogen (BUN) and creatinine, indicating protection against ochratoxin A-induced pathophysiology. Kaempferol also increased phosphorylation of PI3K and AKT, potentially activating antioxidative and anti-apoptotic signaling pathways regulated by Nrf2. Additionally, kaempferol enhanced Bcl-2 activation while suppressing caspase-3, suggesting anti-apoptotic effects. Histopathological analysis revealed reduced renal damage in kaempferol-treated mice compared to those exposed to ochratoxin A alone. Conclusion: Kaempferol demonstrated efficacy in preventing ochratoxin A -induced kidney injury through antioxidative and anti-apoptotic mechanisms. These findings suggest potential therapeutic applications of kaempferol in oxidative stress-induced renal damage. |
| Databáze: | Directory of Open Access Journals |
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