| Autor: |
Theo P. Sloots, Michael D. Nissen, Graeme R. Nimmo, Stephen B. Lambert, Cassandra E. Faux, Cheryl Bletchly, Seweryn Bialasiewicz, David M. Whiley, Rebecca J. Rockett |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Viruses, Vol 1, Iss 3, Pp 1204-1208 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1999-4915 |
| DOI: |
10.3390/v1031204 |
| Popis: |
Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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