iTRAQ-based mass spectrometry screen to identify serum biomarkers in systemic lupus erythematosus
| Autor: | Ting Zhang, Kamala Vanarsa, Ramesh Saxena, Chandra Mohan, C C Mok, Jack Hutcheson, Sneha Ravi Kumar, Satyavani Nukala, Haleigh Inthavong, Bruce Stanley, Tianfu Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Lupus Science and Medicine, Vol 11, Iss 1 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2022-0006 2053-8790 |
| DOI: | 10.1136/lupus-2022-000673 |
| Popis: | Objective Systemic lupus erythematosus (SLE) is a complex systemic autoimmune disorder with no reliable serum biomarkers currently available other than autoantibodies.Methods In the present study, isobaric tags for relative and absolute quantitation-based mass spectrometry was used to screen the sera of patients with SLE to uncover potential disease biomarkers.Results 85 common proteins were identified, with 16 being elevated (≥1.3) and 23 being decreased (≤0.7) in SLE. Of the 16 elevated proteins, serum alpha-1-microglobulin/bikunin precursor (AMBP), zinc alpha-2 glycoprotein (AZGP) and retinol-binding protein 4 (RBP4) were validated in independent cross-sectional cohorts (Cohort I, N=52; Cohort II, N=117) using an orthogonal platform, ELISA. Serum AMBP, AZGP and RBP4 were validated to be significantly elevated in both patients with inactive SLE and patients with active SLE compared with healthy controls (HCs) (p2.5) in Cohort I. All three proteins exhibited good discriminatory power for distinguishing active SLE and inactive SLE (area under the curve=0.82–0.96), from HCs. Serum AMBP exhibited the largest fold change in active SLE (5.96) compared with HCs and correlated with renal disease activity. The elevation in serum AMBP was validated in a second cohort of patients with SLE of different ethnic origins, correlating with serum creatinine (r=0.60, p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|