Tumour necrosis factor-alpha and interleukin-8 inhibit neutrophil migration in vitro and in vivo

Autor: F. Q. Cunha, W. M. S. Cunha Tamashiro
Jazyk: angličtina
Rok vydání: 1992
Předmět:
Zdroj: Mediators of Inflammation, Vol 1, Iss 6, Pp 397-401 (1992)
Druh dokumentu: article
ISSN: 0962-9351
1466-1861
09629351
DOI: 10.1155/S0962935192000607
Popis: Pretreatment of human neutrophils with recombinant tumour necrosis factor-alpha (rTNF-α) and/or interleukin-8 (rIL-8), but not with either transforming growth factor-beta, interleukin-6 or interferon-gamma, rendered these cells less responsive to FMLP, in microchemotaxis assays. This inhibitory effect was dose dependent and more powerful when neutrophils were pretreated with a mixture of both cytokines. Intravenous injection of human rIL-8 (hrIL-8) and/or murine rTNF-α (mrTNF-α) also significantly reduced in vivo neutrophil migration into peritoneal cavities of rats stimulated with carrageenan. These data suggest that the defect in neutrophil migration during septicaemia or endotoxaemia may be the result of the continuous release of IL-8 and TNF-α into the circulation. Thus, either the selective control or blockade of releasing of these cytokines as well as of its effects on neutrophils may be clinically useful in reestablishing the cell defence mechanisms.
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