Establishment of a protocol for storage of refrigerated autologous skin
| Autor: | Renata Oliveira da Conceição, André Oliveira Paggiaro, Eugênio Ferramundo Polo, Karina Martines, Cesar Isaac, Viviane Fernandes de Carvalho, David de Souza Gomez, Rolf Gemperli |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Revista Brasileira de Cirurgia Plástica, Vol 32, Iss 04, Pp 562-569 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1983-5175 2177-1235 |
| DOI: | 10.5935/2177-1235.2017RBCP0091 |
| Popis: | INTRODUCTION: Autologous skin grafts are used for treatment of burn patients. These grafts can be stored and preserved, as long as the storage process is performed with strict quality control to reduce the risk of infection. METHODS: A retrospective cohort study was conducted in the Burn Unit of the Hospital das Clínicas de São Paulo from February 2015 to July 2016. During this period, a protocol was established to store refrigerated skin, with control of collection, preservation, and packaging, and recording of all processes. To ensure quality, graft biopsies were collected for pre- and post-storage microbiology testing and a cross-sectional study for contamination was performed. RESULTS: Critical deficiencies included inadequate packaging, lack of processing records, lack of biopsies for microbiology testing, and failure to discard specimens. Most of the samples were contaminated before and after storage (84.2%). Only two samples were sterile before storage but became contaminated after storage, with growth of Gram-positive skin bacteria. CONCLUSION: A promising method for the storage of refrigerated skin was established, but requires minor adjustments in quality control. |
| Databáze: | Directory of Open Access Journals |
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