Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry

Autor: Eugene B. Postnikov, Michał Wasiak, Mariola Bartoszek, Justyna Polak, Andrey Zyubin, Anastasia I. Lavrova, Mirosław Chora̧żewski
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Molecules, Vol 28, Iss 17, p 6417 (2023)
Druh dokumentu: article
ISSN: 1420-3049
DOI: 10.3390/molecules28176417
Popis: In this work, we review some physical methods of macroscopic experiments, which have been recently argued to be promising for the acquisition of valuable characteristics of biomolecular structures and interactions. The methods we focused on are electron paramagnetic resonance spectroscopy, Raman spectroscopy, and differential scanning calorimetry. They were chosen since it can be shown that they are able to provide a mutually complementary picture of the composition of cellular envelopes (with special attention paid to mycobacteria), transitions between their molecular patterning, and the response to biologically active substances (reactive oxygen species and their antagonists—antioxidants—as considered in our case study).
Databáze: Directory of Open Access Journals
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