Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration

Autor: Arooj Munir, Anne Døskeland, Steven J Avery, Tiziana Fuoco, Samih Mohamed-Ahmed, Henning Lygre, Anna Finne-Wistrand, Alastair J Sloan, Rachel J Waddington, Kamal Mustafa, Salwa Suliman
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Tissue Engineering, Vol 10 (2019)
Druh dokumentu: article
ISSN: 2041-7314
20417314
DOI: 10.1177/2041731419852703
Popis: Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.
Databáze: Directory of Open Access Journals
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