| Autor: |
Yan Ma, Qingjun Li, Yiyun Tang, Zhiyong Zhang, Rongpeng Liu, Qin Luo, Yuting Wang, Jie Hu, Yuqin Chen, Zhiwei Li, Chen Zhao, Yiting Ran, Yuanyuan Mu, Yinghao Li, Xiaoqing Xu, Yuyan Gong, Zihan He, Yongbing Ba, Kaiqi Guo, Keshu Dong, Xiao Li, Wei Tan, Yumeng Zhu, Zhonghuai Xiang, Hanfu Xu |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Cell Reports, Vol 43, Iss 7, Pp 114460- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2211-1247 |
| DOI: |
10.1016/j.celrep.2024.114460 |
| Popis: |
Summary: Natural silks are renewable proteins with impressive mechanical properties and biocompatibility that are useful in various fields. However, the cellular and spatial organization of silk-secreting organs remains unclear. Here, we combined single-nucleus and spatially resolved transcriptomics to systematically map the cellular and spatial composition of the silk glands (SGs) of mulberry silkworms late in larval development. This approach allowed us to profile SG cell types and cell state dynamics and identify regulatory networks and cell-cell communication related to efficient silk protein synthesis; key markers were validated via transgenic approaches. Notably, we demonstrated the indispensable role of the ecdysone receptor (ultraspiracle) in regulating endoreplication in SG cells. Our atlas presents the results of spatiotemporal analysis of silk-secreting organ architecture late in larval development; this atlas provides a valuable reference for elucidating the mechanism of efficient silk protein synthesis and developing sustainable products made from natural silk. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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