Human landing catches provide a useful measure of protective efficacy for the evaluation of volatile pyrethroid spatial repellents

Autor: Mgeni Mohamed Tambwe, Ummi Abdul Kibondo, Olukayode Ganiu Odufuwa, Jason Moore, Ahmed Mpelepele, Rajabu Mashauri, Adam Saddler, Sarah Jane Moore
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Parasites & Vectors, Vol 16, Iss 1, Pp 1-14 (2023)
Druh dokumentu: article
ISSN: 1756-3305
DOI: 10.1186/s13071-023-05685-5
Popis: Abstract Background The human landing catch (HLC) method, in which human volunteers collect mosquitoes that land on them before they can bite, is used to quantify human exposure to mosquito vectors of disease. Comparing HLCs in the presence and absence of interventions such as repellents is often used to measure protective efficacy (PE). Some repellents have multiple actions, including feeding inhibition, whereby mosquitoes may be unable to bite even if they land on a host. A comparison was made between the PE of the volatile pyrethroid spatial repellent (VPSR) transfluthrin determined using a landing method (HLC) and a biting method (allowing the mosquitoes that landed to blood-feed) to evaluate whether HLC is a suitable method for the estimation of the personal PE of a VPSR. Methods A fully balanced, two-arm crossover design study was conducted using a 6 × 6 × 2-m netted cage within a semi-field system. Hessian strips (4 m × 0.1 m) treated with a 5-, 10-, 15-, or 20-g dose of transfluthrin were evaluated against a paired negative control for three strains of laboratory-reared Anopheles and Aedes aegypti mosquitoes. Six replicates were performed per dose using either the landing or the biting method. The number of recaptured mosquitoes was analysed by negative binomial regression, and the PEs calculated using the two methods were compared by Bland–Altman plots. Results For Anopheles, fewer mosquitoes blood-fed in the biting arm than landed in the landing arm (incidence rate ratio = 0.87, 95% confidence interval 0.81–0.93, P
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