| Autor: |
Magdalena Matczak, Anna Marchelak, Piotr Michel, Aleksandra Owczarek, Anna Piszczan, Joanna Kolodziejczyk-Czepas, Paweł Nowak, Monika A. Olszewska |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 40, Iss , Pp 207-218 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2017.10.046 |
| Popis: |
The work combines detailed chemical profiling (UHPLC-PDA-ESI-MS3, HPLC-PDA, UV-spectrophotometric) and in vitro activity testing to provide the first comprehensive characteristics of leaves of the fruit plant S. domestica as a source of bioactive extracts used for the treatment of oxidative stress/inflammation-related pathologies. Polyphenol-rich (44 analytes, total content up to 700.0 ± 19.7 mg/g) extracts, obtained by fractionated extraction, were highly-efficient antioxidants (tests: DPPH, FRAP, TBARS) and anti-inflammatory agents (lipoxygenase and hyaluronidase inhibitors). Moreover, at in vivo-relevant levels (1–5 µg/mL) they effectively protected human plasma components against peroxynitrite-induced oxidative damage (reduced the levels of 3-nitrotyrosine, lipid hydroperoxides and thiobarbituric acid-reactive substances) and normalised/enhanced the total antioxidant status of plasma. Concentration- and phenolic-dependence was clearly evidenced for all models. Furthermore, the extracts were found safe in cytotoxicity test on human PBMCs. The defatted methanol-water (7:3, v/v) extract and its organic solvents fractions were demonstrated as the most advantageous for future applications. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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