MONITORING OF WATERMELON MOSAIC VIRUS 2 IN AGRIECOSYSTEMS OF KYIV AND POLTAVA REGIONS
| Autor: | Т. О. Руднєва, Т. П. Шевченко, В. О. Цвігун, В. П. Поліщук |
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| Jazyk: | English<br />Russian<br />Ukrainian |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Mìkrobìologìâ ì Bìotehnologìâ, Vol 0, Iss 4(24), Pp 55-62 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2076-0558 2307-4663 |
| DOI: | 10.18524/2307-4663.2013.4(24).48976 |
| Popis: | Aim. Monitoring of Watermelon mosaic virus 2 in agriecosystems of Kyiv and Poltava regions. Methods. Plant samples were analyzed using DAS- and indirect ELISA for presence of viral antigens. ELISA samples were prepared by standard methods. For Watermelon mosaic virus 2 (WMV-2) diagnostics, commercial antisera of INRA (France), Agdia (England) and Loewe (Germany) were used. Results. Following 9-year monitoring of Ukrainian agriecosystems we have demonstrated periodically changeable circulation of Watermelon mosaic virus 2 on plants of Cucurbitaceae family. It was determined that, in Ukraine, WMV 2-infected plants were typical only for open ground conditions. Watermelon mosaic virus 2 circulates mainly in form of mixed infection with Zucchini yellow mosaic virus, Cucumber mosaic virus or Cucurbit aphid-born yellows virus. Conclusions. In 2005, after nearly 30 years of absence, WMV-2 has been again detected on plants of Cucurbitaceae family in open-ground agriecosystems of Kyiv and Poltava regions of Ukraine. During the 9-year monitoring, WMV-2 has not been found at all on watermelon plants, though previously this pathogen was widely spread on watermelon plantings. In addition, it should be noticed we have never detected WMV-2 in glasshouse crops. Therefore in Ukraine WMV-2 remains a pathogen infecting exclusively the plants cultivated under open-ground conditions. |
| Databáze: | Directory of Open Access Journals |
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