The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
| Autor: | Michelle F. Schaffer, Guanya Peng, Bernhard Spingler, Joachim Schnabl, Meitian Wang, Vincent Olieric, Roland K. O. Sigel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | International Journal of Molecular Sciences, Vol 17, Iss 7, p 988 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1422-0067 17070988 |
| DOI: | 10.3390/ijms17070988 |
| Popis: | Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to understand the mechanism of metal ions in the catalytic or regulatory activity of RNA. We solved the crystal structure of an octameric RNA duplex in the presence of the di- and trivalent metal ions Ca2+, Mn2+, Co2+, Cu2+, Sr2+, and Tb3+. The detailed investigation reveals a unique innersphere interaction to uracil and extends the knowledge of the influence of metal ions for conformational changes in RNA structure. Furthermore, we could demonstrate that an accurate localization of the metal ions in the X-ray structures require the consideration of several crystallographic and geometrical parameters as well as the anomalous difference map. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|