Genomic characterization of a Brazilian TT Virus isolate closely related to SEN virus-F
| Autor: | Leonardo Diniz-Mendes, Sylvie Devalle, Christian Niel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 99, Iss 3, Pp 301-306 (2004) |
| Druh dokumentu: | article |
| ISSN: | 0074-0276 1678-8060 |
| DOI: | 10.1590/S0074-02762004000300011 |
| Popis: | SEN virus (SENV) is a circular, single stranded DNA virus that has been first characterized in the serum of a human immunodeficiency virus type 1 (HIV-1)-infected patient. Eight genotypes of SENV (A-H) have been identified and further recognized as variants of TT virus (TTV) in the family Circoviridae. Here we describe the first genomic characterization of a SENV isolate (5-A) from South America. Using 'universal' primers, able to amplify most, if not all, TTV/SENV genotypes, a segment of > 3 kb was amplified by polymerase chain reaction from the serum of an HIV-1 infected patient. The amplicon was cloned and a 3087-nucleotide sequence was determined, that showed a high (85%) homology with the sequence of the Italian isolate SENV-F. Proteins encoded by open reading frames (ORFs) 1 to 4 consisted of 758, 129, 276, and 267 amino acids, respectively. By phylogenetic analysis, isolate 5-A was classified into TTV genotype 19 (phylogenetic group 3), together with SENV-F and TTV isolate SAa-38. |
| Databáze: | Directory of Open Access Journals |
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