Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
| Autor: | Nuno Palha, Florence Guivel-Benhassine, Valérie Briolat, Georges Lutfalla, Marion Sourisseau, Felix Ellett, Chieh-Huei Wang, Graham J Lieschke, Philippe Herbomel, Olivier Schwartz, Jean-Pierre Levraud |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | PLoS Pathogens, Vol 9, Iss 9, p e1003619 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1553-7366 1553-7374 |
| DOI: | 10.1371/journal.ppat.1003619 |
| Popis: | Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of ∼14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus. |
| Databáze: | Directory of Open Access Journals |
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