PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO
| Autor: | Edgar Antonio Reyes Montaño, Leonardo Lareo, Gerardo Pérez |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Revista Colombiana de Química, Vol 41, Iss 1, Pp 31-45 (2011) |
| Druh dokumentu: | article |
| ISSN: | 0120-2804 2357-3791 |
| Popis: | Producing polyclonal antibodies (IgY) inchickens has advantages over those obtainedin other animal models, since theyhave been used as a tool for studyingdifferent proteins (NMDA glutamate receptorin our case, specifically the NR1subunit). We produced specific antibodiesagainst expression products by thealternative splicing of the gene encodingNMDA receptor NR1 subunit in adult ratbrain. Three peptides corresponding tothe splicing sites (N1, C1 and C2’ cassettes)were designed, synthesised and usedindividually as antigens in hens. Specificimmunoglobulins were purified fromyolks. The antibodies were then used forpurifying the NMDA receptor NR1 subunitusing affinity chromatography couplingthe three antibodies to the support.R |
| Databáze: | Directory of Open Access Journals |
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