| Autor: |
Zachary Richards, Tara McCray, Joseph Marsili, Morgan L. Zenner, Jacob T. Manlucu, Jason Garcia, Andre Kajdacsy-Balla, Marcus Murray, Cindy Voisine, Adam B. Murphy, Sarki A. Abdulkadir, Gail S. Prins, Larisa Nonn |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
iScience, Vol 12, Iss , Pp 304-317 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2589-0042 |
| DOI: |
10.1016/j.isci.2019.01.028 |
| Popis: |
Summary: The fibromuscular stroma of the prostate regulates normal epithelial differentiation and contributes to carcinogenesis in vivo. We developed and characterized a human 3D prostate organoid co-culture model that incorporates prostate stroma. Primary prostate stromal cells increased organoid formation and directed organoid morphology into a branched acini structure similar to what is observed in vivo. Organoid branching occurred distal to physical contact with stromal cells, demonstrating non-random branching. Stroma-induced phenotypes were similar in all patients examined, yet they maintained inter-patient heterogeneity in the degree of response. Stromal cells expressed growth factors involved in epithelial differentiation, which was not observed in non-prostatic fibroblasts. Organoids derived from areas of prostate cancer maintained differential expression of alpha-methylacyl-CoA racemase and showed increased viability and passaging when co-cultured with stroma. The addition of stroma to epithelial cells in vitro improves the ability of organoids to recapitulate features of the tissue and enhances the viability of organoids. : Bioengineering; Biological Sciences; Cell Biology; Tissue Engineering Subject Areas: Bioengineering, Biological Sciences, Cell Biology, Tissue Engineering |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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