Comparison of ruminal microbiota, IL-1β gene variation, and tick incidence between Holstein × Gyr and Holstein heifers in grazing system

Autor: Daiana Francisca Quirino, Marcos Inácio Marcondes, Kellen Ribeiro de Oliveira, Simone Elisa Facioni Guimarães, Juliana Soares da Silva, Garret Suen, Letícia Elisa Rossi, Camila Soares Cunha, Hilario Cuquetto Mantovani, Polyana Pizzi Rotta
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Frontiers in Microbiology, Vol 15 (2024)
Druh dokumentu: article
ISSN: 1664-302X
DOI: 10.3389/fmicb.2024.1132151
Popis: IntroductionThe variation in bacterial communities among breeds has been previously reported and may be one of the reasons why Holstein × Gyr dairy heifers have better development in grazing systems in tropical conditions. This study aimed to explore the ruminal microbiota composition, the IL-1β gene variation, tick incidence, and blood parameters of Holstein × Gyr (½ Holstein × ½ Gyr) and Holstein heifers grazing intensely managed Guinea grass (Panicum maximum Jacq. cv. Mombaça).MethodsSixteen heifers were divided into two groups consisting of 8 Holstein × Gyr and 8 Holstein heifers. The experimental period was comprised of 3 periods of 21 days. Ruminal samples were taken via the stomach tube technique. The sequencing of the V4 hypervariable region of the 16S rRNA gene was performed using the Illumina MiSeq platform. Counting and collection of ticks were conducted each 21 days. Blood and skeletal muscle tissue biopsies were performed at the end of the experiment.ResultsFirmicutes were the most abundant phyla present in both breed rumen samples and Bacteroidota showed differences in relative abundance between breed groups, with greater values for Holstein heifers (p
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