Comparative Evaluation of Cytotoxicity of 0.12% and 0.2% Chlorhexidine, 2% Povidone Iodine, 3% Hydrogen Peroxide and 0.9% Normal Saline Solutions on Fibroblasts- An Invitro Study
| Autor: | Sruthy Madhusudanan, Ambili Renjith |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 14, Iss 9, Pp ZC21-ZC25 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2249-782X 0973-709X |
| DOI: | 10.7860/JCDR/2020/44500.14037 |
| Popis: | Introduction: Periodontitis is initiated by plaque microbes and modified by systemic and environmental factors. Treatment of periodontitis primarily focuses on plaque control by mechanical and chemical means. Chlorhexidine (CHX) mouthwash is considered as the ‘gold standard’ chemical plaque control agent. But studies have demonstrated cytotoxic effects of CHX. However, there is limited evidence available regarding the cytotoxicity of other commonly used postoperative mouthwashes. Aim: To evaluate cytotoxicity of commonly used postoperative mouthwashes (CHX- 0.12% and 0.2%, 2% povidone iodine, 3% hydrogen peroxide and 0.9% normal saline solutions) using MTT assay on fibroblast cells and to identify the least cytotoxic agent. Materials and Methods: The study was an invitro study conducted at Department of Periodontics, PMS College of Dental Sciences and Research, Vattapara, Thiruvananthapuram in association with Biogenix research centre Poojapura in January 2018. The cytotoxic effects of CHX -0.12% and 0.2%, Povidone iodine 2%, 3% hydrogen peroxide and 0.9% normal saline solution on L929 fibroblast cells were observed using inverted phase contrast microscope and images were recorded for all the groups. Cytotoxic evaluation was done by MTT {3,(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide} assay. Optical Density (OD) was measured and percentage of cell viability for each mouthwash was calculated. Statistical Analysis was carried out using analysis of variance (ANOVA). Intergroup comparison was done using Post hoc analysis (Tukey HSD). The p-value |
| Databáze: | Directory of Open Access Journals |
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