Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure
| Autor: | Monika Kremplova, Dana Fialova, Lukas Nejdl, David Hynek, Libuse Trnkova, Jaromir Hubalek, Rene Kizek, Vojtech Adam |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
adenine
adenine interaction magnetic beads square wave voltammetry aptamer biosensor nanobiotechnology Technology Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 |
| Zdroj: | Materials, Vol 7, Iss 3, Pp 1455-1472 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma7031455 |
| Popis: | The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected—peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too. |
| Databáze: | Directory of Open Access Journals |
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