The Utilization of Vegetable and Fruit Wastes for Saccharomyces cerevisieae Cell Wall Based β-Glucan Production with Antioxidant Activity
| Autor: | Gemilang Lara Utama, Florensia Irena, Elazmanawati Lembong, Indira Lanti Kayaputri, Tensiska Tensiska, Roostita Lobo Balia |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Vol 68, Iss 1, Pp 119-127 (2020) |
| Druh dokumentu: | article |
| ISSN: | 20206801 1211-8516 2464-8310 |
| DOI: | 10.11118/actaun202068010119 |
| Popis: | The study aims to determine the number of mass and antioxidant activity of β-glucan extracted from S.cerevisieae which grown on vegetable and fruit wastes. The method used is experimental with descriptive analysis which consisted of 3 treatments namely banana waste, papaya waste, and napa cabbage waste as fermentation medium, repeated thrice. Fermentation medium was made by mixing waste with water with the ratio of 1:2. Ten percent (w/v) of S.cerevisieae was inoculated and incubated for 48 hours, at 27 ℃. Extraction of β-glucan was carried out using acid-alkaline methods and antioxidant activity was tested by DPPH (2,2-Diphenyl-1-picrylhydrayl) method and the microstructure of β-glucan is determined by Scanning Electrone Microscope. The result showed that the best medium in producing β-glucan was papaya waste which resulting 19.094 g β-glucan mass, with radical scavenging activity of 20.71 % and globular diameter of 533 μm. |
| Databáze: | Directory of Open Access Journals |
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