Evaluation of micronuclei in tobacco users: A study in Punjabi population
| Autor: | Himanta Bansal, V Simarpreet Sandhu, Rajat Bhandari, Deepti Sharma |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Contemporary Clinical Dentistry, Vol 3, Iss 2, Pp 184-187 (2012) |
| Druh dokumentu: | article |
| ISSN: | 0976-237X 0976-2361 |
| DOI: | 10.4103/0976-237X.96825 |
| Popis: | Introduction: The assessment of micronuclei in exfoliated cells is a promising tool for the study of epithelial carcinogens and can be used to detect chromosome breakage or mitotic interference, thought to be relevant to carcinogenesis. Aim: The present study aimed to detect micronuclei in exfoliated oral mucosal cells in individuals using various tobacco forms from the last 5 years. Materials and Methods: A total of 75 healthy male subjects (25 smokeless tobacco users, 25 smokers, and 25 non-tobacco users) were selected for the study. Smears were obtained with moistened wooden spatula from buccal mucosa and fixed with 95% alcohol. All the cytologic smears were stained by Papanicolaou technique. From each slide, ~1000 cells were examined under the 400× magnification and where micronucleated (MN) cells were located, they were examined under the 1000× magnification. Result: MN cells were found to be significantly higher in smokeless tobacco users than in smokers and controls. Conclusion: A positive correlation is found between increased micronucleus frequency and tobacco-using habits. So micronucleus assay can be used as a biomarker of genotoxicity. |
| Databáze: | Directory of Open Access Journals |
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