| Autor: |
Domenico Lo Tartaro, Antonio Camiro-Zúñiga, Milena Nasi, Sara De Biasi, Marco A. Najera-Avila, Maria Del Rocio Jaramillo-Jante, Lara Gibellini, Marcello Pinti, Anita Neroni, Cristina Mussini, Luis E. Soto-Ramírez, Juan J. Calva, Francisco Belaunzarán-Zamudio, Brenda Crabtree-Ramirez, Christian Hernández-Leon, Juan L. Mosqueda-Gómez, Samuel Navarro-Álvarez, Santiago Perez-Patrigeon, Andrea Cossarizza |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cells, Vol 11, Iss 15, p 2307 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2073-4409 |
| DOI: |
10.3390/cells11152307 |
| Popis: |
Several studies have identified main changes in T- and B-lymphocyte subsets during chronic HIV infection, but few data exist on how these subsets behave during the initial phase of HIV infection. We enrolled 22 HIV-infected patients during the acute stage of infection before the initiation of antiretroviral therapy (ART). Patients had blood samples drawn previous to ART initiation (T0), and at 2 (T1) and 12 (T2) months after ART initiation. We quantified cellular HIV-DNA content in sorted naïve and effector memory CD4 T cells and identified the main subsets of T- and B-lymphocytes using an 18-parameter flow cytometry panel. We identified correlations between the patients’ clinical and immunological data using PCA. Effective HIV treatment reduces integrated HIV DNA in effector memory T cells after 12 months (T2) of ART. The main changes in CD4+ T cells occurred at T2, with a reduction of activated memory, cytolytic and activated/exhausted stem cell memory T (TSCM) cells. Changes were present among CD8+ T cells since T1, with a reduction of several activated subsets, including activated/exhausted TSCM. At T2 a reduction of plasmablasts and exhausted B cells was also observed. A negative correlation was found between the total CD4+ T-cell count and IgM-negative plasmablasts. In patients initiating ART immediately following acute/early HIV infection, the fine analysis of T- and B-cell subsets has allowed us to identify and follow main modifications due to effective treatment, and to identify significant changes in CD4+ and CD8+ T memory stem cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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