Skin treatment with non-thermal plasma modulates the immune system through miR-223-3p and its target genes

Autor: Annika Engel, Nicole Ludwig, Friederike Grandke, Viktoria Wagner, Fabian Kern, Tobias Fehlmann, Georges P. Schmartz, Ernesto Aparicio-Puerta, Dominic Henn, Barbara Walch-Rückheim, Matthias Hannig, Stefan Rupf, Eckart Meese, Matthias W. Laschke, Andreas Keller
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: RNA Biology, Vol 21, Iss 1, Pp 31-44 (2024)
Druh dokumentu: article
ISSN: 15476286
1555-8584
1547-6286
DOI: 10.1080/15476286.2024.2361571
Popis: Non-thermal plasma, a partially ionized gas, holds significant potential for clinical applications, including wound-healing support, oral therapies, and anti-tumour treatments. While its applications showed promising outcomes, the underlying molecular mechanisms remain incompletely understood. We thus apply non-thermal plasma to mouse auricular skin and conducted non-coding RNA sequencing, as well as single-cell blood sequencing. In a time-series analysis (five timepoints spanning 2 hours), we compare the expression of microRNAs in the plasma-treated left ears to the unexposed right ears of the same mice as well as to the ears of unexposed control mice. Our findings indicate specific effects in the treated ears for a set of five miRNAs: mmu-miR-144-5p, mmu-miR-144-3p, mmu-miR-142a-5p, mmu-miR-223-3p, and mmu-miR-451a. Interestingly, mmu-miR-223-3p also exhibits an increase over time in the right non-treated ear of the exposed mice, suggesting systemic effects. Notably, this miRNA, along with mmu-miR-142a-5p and mmu-miR-144-3p, regulates genes and pathways associated with wound healing and tissue regeneration (namely ErbB, FoxO, Hippo, and PI3K-Akt signalling). This co-regulation is particularly remarkable considering the significant seed dissimilarities among the miRNAs. Finally, single-cell sequencing of PBMCs reveals the downregulation of 12 from 15 target genes in B-cells, Cd4+ and Cd8+ T-cells. Collectively, our data provide evidence for a systemic effect of non-thermal plasma.
Databáze: Directory of Open Access Journals
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