PROTEOLYTIC DEGRADATION OF POLY (ADP-RIBOSE) POLYMERASE IN RATS WITH CARRAGEENAN-INDUCED GASTROENTEROCOLITIS
| Autor: | Tkachenko A. S., Nakonechna O. A., Zhukov V. I., Gorbach T. V., Tkachenko M. O. |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Journal of V. N. Karazin Kharkiv National University: Series Medicine, Iss 34, Pp 41-46 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2313-6693 2313-2396 |
| Popis: | The aim of the research was to study the activity of poly (ADP-ribose) polymerase in small intestinal homogenate of rats with chronic carrageenan-induced gastroenterocolitis, as well as mechanisms of regulation of the enzyme in this pathology. Twenty Wistar Albino Glaxo rats were divided into two groups. Animals of group 1 (n = 10) consumed 1 % carrageenan solution for 28 days, which resulted in the development of gastroenterocolitis confirmed morphologically. The control group consisted of intact animals (n = 10). The activity of poly (ADP-ribose) polymerase (PARP) in the homogenate of small intestine, as well as caspase-3, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) serum levels were determined. Obtained data were statistically processed using the Mann-Whitney U test and calculating median and interquartile range (Me, 25th–75th percentile) with the help of the GraphPad Prism 5 application. The development of carrageenan-induced gastroenterocolitis was accompanied by an increase in caspase-3, MMP-2, MMP-9 concentrations in blood serum and a decrease in the activity of PARP in small intestinal homogenates. The reduced activity of PARP in chronic carrageenan-induced gastroenterocolitis may be due to the proteolysis of this enzyme under the action of caspase-3, MMP-2, and MMP-9. |
| Databáze: | Directory of Open Access Journals |
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