| Autor: |
Chien-I Chang, Kimberly A. Schandel, Duane D. Jenness |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Biology Open, Vol 3, Iss 4, Pp 297-306 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2046-6390 |
| DOI: |
10.1242/bio.20146866 |
| Popis: |
This study investigates endocytosis of Saccharomyces cerevisiae α-factor receptor and the role that receptor oligomerization plays in this process. α-factor receptor contains signal sequences in the cytoplasmic C-terminal domain that are essential for ligand-mediated endocytosis. In an endocytosis complementation assay, we found that oligomeric complexes of the receptor undergo ligand-mediated endocytosis when the α-factor binding site and the endocytosis signal sequences are located in different receptors. Both in vitro and in vivo assays suggested that ligand-induced conformational changes in one Ste2 subunit do not affect neighboring subunits. Therefore, recognition of the endocytosis signal sequence and recognition of the ligand-induced conformational change are likely to be two independent events. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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