The Upregulation of Carnitine Palmitoyltransferase 1a (CPT1a) Expression under Prolonged Fasting in CD36 Knockout Mice
| Autor: | Mirasari Putri, Mas Rizky A.A. Syamsunarno, Tatsuya Iso, Masahiko Kurabayashi |
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| Jazyk: | English<br />Indonesian |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Global Medical & Health Communication, Vol 6, Iss 2, Pp 84-87 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2301-9123 2460-5441 |
| DOI: | 10.29313/gmhc.v6i2.3286 |
| Popis: | Food deprivation is one of the extreme conditions that mammals have to survive. The majority of the tissues, excluding the brain and red blood cells, depend on the fatty acids (FA) utilization to produce energy. We recently showed in mice lacking for CD36 (CD36−/−), the uptake of FA is limited with dramatically increased of glucose uptake in heart and skeletal muscle in fasted condition, indicating a compensatory mechanism of organ to fulfill an energy demand. The liver is the central tissue maintaining metabolic homeostasis in fasted state. Synthesize adenosine triphosphate (ATP) in the mitochondria via beta-oxidation was mediated by carnitine palmitoyltransferase 1a (CPT1a). The objective of this research was to explore the role of CD36 in CPT1a expression in the fasted state. This research was conducted at Gunma University Japan in 2015. The method was in vivo-experimental, that we used CD36−/− and wild-type (WT) mice, as a control. The gene expression of CPT1a was measured by real-time PCR. Fasting condition up regulated mRNA expression of CPT1a in both WT and CD36−/− mice in 24 h and 48 h. However in CD36−/− mice, the mRNA expression of CPT1a in 24 h fasted state was lower very significantly than WT mice (p |
| Databáze: | Directory of Open Access Journals |
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