Highly Effective DNA Extraction Method from Fresh, Frozen, Dried and Clotted Blood Samples
| Autor: | Jaleh Barar, Sina Atashpaz, Abolfazl Barzegari, Vala Kafil, Sepideh Zununi Vahed, Farzaneh Soltanzad, Sara Samadi Shams |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | BioImpacts, Vol 1, Iss 3, Pp 183-187 (2011) |
| Druh dokumentu: | article |
| ISSN: | 2228-5652 2228-5660 |
| DOI: | 10.5681/bi.2011.025 |
| Popis: | Introduction: Today, with the tremendous potential of genomics and other recent advances in science, the role of science to improve reliable DNA extraction methods is more relevant than ever before. The ideal process for genomic DNA extraction demands high quantities of pure, integral and intact genomic DNA (gDNA) from the sample with minimal co-extraction of inhibitors of downstream processes. Here, we report the development of a very rapid, less-hazardous, and high throughput protocol for extracting of high quality DNA from blood samples. Methods: Dried, clotted and ethylene diamine tetra-acetic acid (EDTA) treated fresh and frozen blood samples were extracted using this method in which the quality and integrity of the extracted DNA were corroborated by agarose gel electrophoresis, PCR reaction and DNA digestion using restricted enzyme. The UV spectrophotometric and gel electrophoresis analysis resulted in high A260/A280 ratio (>1.8) with high intactness of DNA. Results: PCR and DNA digestion experiments indicated that the final solutions of extracted DNA contained no inhibitory substances, which confirms that the isolated DNA is of good quality. Conclusion: The high quality and quantity of current method, no enzymatic processing and accordingly its low cost, make it appropriate for DNA extraction not only from human but also from animal blood samples in any molecular biology labs. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|