Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar

Autor: Nabuqi, Nuoendagula, Sejirihu Wu, Naoki Takata, Shingo Sakamoto, Masanobu Yamamoto, Mikiko Uesugi, Annabelle Déjardin, Gilles Pilate, Toru Taniguchi, Nobutaka Mitsuda, Shinya Kajita
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Wood Science, Vol 66, Iss 1, Pp 1-9 (2020)
Druh dokumentu: article
ISSN: 1435-0211
1611-4663
DOI: 10.1186/s10086-020-01902-2
Popis: Abstract Increasing the wood density to a preferred range contributes to upgrading the value of wood as raw material. Lignin manipulation can also improve wood quality. This study attempted to accelerate secondary cell wall formation in transgenic poplar with an altered lignin structure. To achieve this, OsSWN1, a rice master switch for secondary cell wall formation in fiber cells, was overexpressed in poplar plants in which monolignol biosynthesis was suppressed by the expression of an RNA-interference construct targeted to the gene for cinnamyl alcohol dehydrogenase 1. The generated transgenic poplars successfully overexpressed the chimeric OsSWN1 construct and monolignol biosynthesis remained altered in these plants. Secondary cell wall thickness was increased in the transgenic plants and their wood density was higher compared to the background line. No difference in lignin content was observed, except in one transgenic line. High saccharification characteristics observed in the background line were preserved in the transgenic line with lower OsSWN1 overexpression, but higher OsSWN1 overexpression had a slight negative impact on enzyme saccharification. Our data suggested that fine-tuning of accelerated deposition of the secondary cell wall combined with alteration of monolignol biosynthesis should improve the lignocellulose quality for conventional and future biorefinery uses.
Databáze: Directory of Open Access Journals
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