Use of ATP luminometry to assess the cleanliness of equipment used to collect and feed colostrum on dairy farms

Autor: Sébastien Buczinski, Marie-Pascale Morin, Jean-Philippe Roy, Marjolaine Rousseau, Marianne Villettaz-Robichaud, Jocelyn Dubuc
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Dairy Science, Vol 105, Iss 2, Pp 1638-1648 (2022)
Druh dokumentu: article
ISSN: 0022-0302
DOI: 10.3168/jds.2021-21023
Popis: ABSTRACT: The objective of this observational cross-sectional study was to describe the cleanliness of various equipment used for colostrum harvest and calf feeding procedures on dairy farms in Québec, Canada. The study was performed on 42 commercial dairy herds also enrolled in another study aiming to determine the transfer of passive immunity over a 14-mo period. Information on colostrum quality (using Brix value) and cleanliness (total aerobic and total coliform count) were recorded as well as various practices focused on colostrum-feeding equipment and preweaning period using a standard questionnaire. During the study period, colostrum and milk-feeding equipment cleanliness was assessed using direct surface swabbing with Hygiena Ultrasnap swabs. A total of 155 swab samples were obtained from 6 pieces of equipment. Adenosine triphosphate collected from the swabbed surface reacts with the luciferase solution present in the swab by bioluminescence, which is proportional to the quantity of ATP present and quantified as relative light units (RLU). The description of feed equipment cleanliness (defined as the maximal RLU found for a specific herd, dichotomized as
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