Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses
| Autor: | Luiz Fernando Frascino, Luca Reis Frascino, Jorge Alberto Thome, Moacir Fernandes de Godoy |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Revista Brasileira de Cirurgia Plástica, Vol 39, Iss 1 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1983-5175 2177-1235 |
| DOI: | 10.5935/2177-1235.2023RBCP0799-EN |
| Popis: | Introduction: Histological analysis is the main tool for evaluating acellular bioprostheses, mostly on an experimental basis. The objective is to histologically analyze the acellular matrix of bovine pericardium in abdominal wall repairs implanted in humans. Method: From a series of 30 repairs with the membrane, 3 patients underwent surgical revision unrelated to the implants at 13, 22, and 23 months postoperatively, obtaining biopsies of the previously implanted areas. In addition to evaluating the basic aspects of biocompatibility and tissue neoformation, the slides were digitalized and subjected to computerized analysis with the ImageJ software to quantify the kinetics of membrane degradation associated with the analysis of the fractal dimension of the samples. The values obtained for percentages of residual membrane had their means compared by analysis of variance (ANOVA) and the unpaired Student’s T test, also used for the fractal dimension quantification values. Results: The biocompatibility of the material was demonstrated, with tissue neoformation, collagen deposition, and cellularized tissue with a normal appearance without important local reactions. Residual fragments of the membrane were quantified at 40%±7% at 13 months, at 20%±6% at 22 months, and at 17%±6% at 23 months postoperatively, with the analysis of the fractal dimension indicating a progressive degradation of implants, with statistical significance between 13 months and late samples. Conclusion: The results confirmed the functionality of the acellular bovine pericardium under different levels of mechanical stress in abdominal wall repairs in humans. |
| Databáze: | Directory of Open Access Journals |
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