Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

Autor:	Fabiola Mancini, Fabrizio Barbanti, Maria Scaturro, Giulia Errico, Angelo Iacobino, Antonino Bella, Flavia Riccardo, Giulia Marsili, Paola Stefanelli, Patrizio Pezzotti, Giovanni Rezza, Alessandra Ciervo
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	SARS-CoV-2 COVID 19 rt-Real time PCR molecular methods heat treatment nasopharyngeal samples Infectious and parasitic diseases RC109-216 Microbiology QR1-502
Zdroj:	Emerging Microbes and Infections, Vol 9, Iss 1, Pp 1393-1396 (2020)
Druh dokumentu:	article
ISSN:	22221751 2222-1751
DOI:	10.1080/22221751.2020.1775500
Popis:	ABSTRACTThe RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/0f0ba7dc6fa9444a8954302830ca4185 Zobrazit plný text záznamu View record in DOAJ