| Popis: |
7 lectins with different carbohydrate affinities (SNA, WGA, RCA, UDA, SBA, HPA and PNA) were used forhistochemical investigation of sialoglycans and related oligosaccharidic determinants in rat ovary and endometrium underexperimental hypo- and hyperthyroidism. Tissue samples were fixed in 4% neutral formalin and embedded in paraffin. Lectin-peroxidase labels were visualized by diaminobenzidine. Both hypo- and hyperthyroidism significantly influenced ovarianand endometrial glycopolymers: rearrangement of NeuNAc, DGlcNAc, DGalNAc and DGal determinants in each structuralcomponent demonstrated specific and differential manifestations. It was detected that hyperthyroidism in comparison withhypothyroidism influenced carbohydrates of female reproductive system more strongly; endometrium was more susceptibleto thyroxin-modulated impairments in comparison with ovaria. Treatment of hypothyroidism with thyroxin normalized leucocytecount, quantitative and qualitative characteristics of collagen fibers and expression of sialoglycans, but not SBA, HPAand PNA receptor sites. Selective labeling of zona pellucida and corona radiata, luteocytes, atretic follicles and leucocyteswas detected in ovaria; in endometrium lectin label was attributed to leucocytes and collagen fibers, epithelium and secretorycomponents of uterine glands. |
