Manufacturing of biodegradable polyurethane scaffolds based on polycaprolactone using a phase separation method: physical properties and in vitro assay
| Autor: | Asefnejad A, Khorasani MT, Behnamghader A, Farsadzadeh B, Bonakdar S |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | International Journal of Nanomedicine, Vol 2011, Iss default, Pp 2375-2384 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1176-9114 1178-2013 |
| Popis: | Azadeh Asefnejad1, Mohammad Taghi Khorasani2, Aliasghar Behnamghader3, Babak Farsadzadeh1, Shahin Bonakdar4 1Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran; 2Iran Polymers and Petrochemical Institute, Tehran, Iran; 3Materials and Energy Research Center, Tehran, Iran; 4National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran Background: Biodegradable polyurethanes have found widespread use in soft tissue engineering due to their suitable mechanical properties and biocompatibility. Methods: In this study, polyurethane samples were synthesized from polycaprolactone, hexamethylene diisocyanate, and a copolymer of 1,4-butanediol as a chain extender. Polyurethane scaffolds were fabricated by a combination of liquid–liquid phase separation and salt leaching techniques. The effect of the NCO:OH ratio on porosity content and pore morphology was investigated. Results: Scanning electron micrographs demonstrated that the scaffolds had a regular distribution of interconnected pores, with pore diameters of 50–300 µm, and porosities of 64%–83%. It was observed that, by increasing the NCO:OH ratio, the average pore size, compressive strength, and compressive modulus increased. L929 fibroblast and chondrocytes were cultured on the scaffolds, and all samples exhibited suitable cell attachment and growth, with a high level of biocompatibility. Conclusion: These biodegradable polyurethane scaffolds demonstrate potential for soft tissue engineering applications. Keywords: polyurethane, tissue engineering, biodegradable, fibroblast cells |
| Databáze: | Directory of Open Access Journals |
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