Histologic and inflammatory lamellar changes in horses with oligofructose-induced laminitis treated with a CXCR1/2 antagonist
| Autor: | Leonardo R. de Lima, Heloisa M.F. Mendes, Frederico M. Soriani, Danielle G. de Souza, Geraldo Eleno S. Alves, Mauro M. Teixeira, Rafael R. Faleiros |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Pesquisa Veterinária Brasileira, Vol 36, Iss 1, Pp 13-18 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1678-5150 0100-736X |
| DOI: | 10.1590/S0100-736X2016000100002 |
| Popis: | Abstract: With the hypothesis that blocking chemokine signaling can ameliorate acute laminitis, the aim was to evaluate the therapeutic effect of intravenous DF1681B, a selective antagonist for CXCR1 and CXCR2 (chemokine receptors), in an oligofructose equine laminitis model. To twelve mixed breed clinically healthy hoses with no previous history of hoof-related lameness was administered oligofructose (10g/kg given by nasogastric tube) and divided into two groups: treated (intravenous DF1681B at 30mg/kg 6, 12, 18, and 24h after oligofructose) and non-treated groups. Laminar biopsies were performed before and 12, 36, and 72h after administering oligofructose. Samples were stained with periodic acid-Schiff (PAS) and scored from 0 to 6 according to epidermal cell and basal membrane changes. The IL-1β, IL-6, and CXCL1 RNA expressions were determined by RT-PCR. Parametric and non-parametric tests were used to compare times within each group (P |
| Databáze: | Directory of Open Access Journals |
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