Survivin and caspases serum protein levels and survivin variants mRNA expression in sepsis

Autor: Marianna Miliaraki, Panagiotis Briassoulis, Stavroula Ilia, Aikaterini Polonifi, Marina Mantzourani, Efrossini Briassouli, Konstantinos Vardas, Serafim Nanas, Aikaterini Pistiki, Maria Theodorakopoulou, Theonymfi Tavladaki, Anna Maria Spanaki, Eumorfia Kondili, Helen Dimitriou, Sotirios Tsiodras, Dimitrios Georgopoulos, Apostolos Armaganidis, George Daikos, George Briassoulis
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-020-78208-2
Popis: Abstract Sepsis is a dysregulated host response to infection related to devastating outcomes. Recently, interest has been shifted towards apoptotic and antiapoptotic pathobiology. Apoptosis is executed through the activation of caspases regulated by a number of antiapoptotic proteins, such as survivin. The survivin and caspases’ responses to sepsis have not yet been elucidated. This is a multicenter prospective observational study concerning patients with sepsis (n = 107) compared to patients with traumatic systemic inflammatory response syndrome (SIRS) (n = 75) and to healthy controls (n = 89). The expression of survivin was quantified through real-time quantitative polymerase chain reaction for the different survivin splice variants (wild type-WT, ΔEx3, 2B, 3B) in peripheral blood leukocytes. The apoptotic or antiapoptotic tendency was specified by measuring survivin-WT, caspase-3, and -9 serum protein concentrations through enzyme-linked immunosorbent assay. The survivin-WT, -2B, -ΔΕx3 mRNA, survivin protein, and caspases showed an escalated increase in SIRS and sepsis, whereas survivin-3B was repressed in sepsis (p
Databáze: Directory of Open Access Journals
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