Light microscopy based approach for mapping connectivity with molecular specificity

Autor: Fred Y. Shen, Margaret M. Harrington, Logan A. Walker, Hon Pong Jimmy Cheng, Edward S. Boyden, Dawen Cai
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-020-18422-8
Popis: Mapping neuroanatomy is a foundational goal of connectomics, and the gold standard method is electron microscopy as light microscopy lacks nanoscale resolution. Here the authors develop a strategy using multicolor genetic labeling (Brainbow) and expansion microscopy to map putative synaptic connections using light microscopy.
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