Traceability of PDO Olive Oil 'Terra di Bari' Using High Resolution Melting
| Autor: | Cinzia Montemurro, Monica Marilena Miazzi, Antonella Pasqualone, Valentina Fanelli, Wilma Sabetta, Valentina di Rienzo |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of Chemistry, Vol 2015 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2090-9063 2090-9071 |
| DOI: | 10.1155/2015/496986 |
| Popis: | The aim of the research was to verify the applicability of microsatellite (SSR) markers in High Resolution Melting (HRM) analysis for the identification of the olive cultivars used in the “Terra di Bari” PDO extra virgin olive oil. A panel of nine cultivars, widespread in Apulia region, was tested with seventeen SSR primer pairs and the PCR products were at first analysed with a Genetic Analyzer automatic sequencer. An identification key was obtained for the nine cultivars, which showed an unambiguous discrimination among the varieties constituting the “Terra di Bari” PDO extra virgin olive oil: Cima di Bitonto, Coratina, and Ogliarola. Subsequently, an SSR based method was set up with the DCA18 marker, coupled with HRM analysis for the distinction of the Terra di Bari olive oil from non-Terra di Bari olive oil using different mixtures. Thus, this analysis enabled the distinction and identification of the PDO mixtures. Hence, this assay provided a flexible, cost-effective, and closed-tube microsatellite genotyping method, well suited to varietal identification and authentication analysis in olive oil. |
| Databáze: | Directory of Open Access Journals |
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